post-planting bionematicide scientific results

Cedroz has been proven effective against root-knot nematode Meloidogyne incognita in various studies. These include in vitro bioassays conducted by the Emilia-Romagna region Plant Protection Service under Dr. Giovanna Curto; preliminary trials on young tomato plants at Ghent University; evaluations on mature egg mass on tomato plant roots by the Emilia-Romagna region Plant Protection Service; and semicommercial field trials on cucumber, tomato and bell pepper.

A row of growing baby tomato plants

In vitro bioassays

In vitro bioassay of Cedroz versus Meloidogyne incognita

The objective of the in vitro bioassay was to verify the nematicidal and/or nematistatic efficacy of Cedroz against the larvae of second-stage juveniles (L2) of the root-knot nematode Meloidogyne incognita.

Step 1: Inoculum production (L2)

  • Mature egg mass from roots of tomato plants of the farms of the Emilia-Romagna region of Italy
  • Dipped in water for seven days to obtain peers of seven-day-old larvae

Step 2: Placement of the L2 larvae in the wells

  • Ten to 12 L2 in 1 mL of aqueous solution of Cedroz
  • Assayed concentrations (mL/L): 2–2, 50–4, 0–5.00
  • The wells were closed with a glass lid and sealed with silicone to contain the dispersion of volatile substances and incubate at room temperature.
  • In each test, three replicates were performed for each concentration and for each test time (24–48 hr). A total of 24 wells were set up, including witnesses (UTC).

Step 3: Assessments — vitality of the larvae L2

  1. Direct observation under a stereomicroscope after 24 and 48 hours of immersion in aqueous solution of Cedroz
  2. The immobile larvae were transferred to distilled water and observed after 24 and 48 hours.
    • Recovery of mobility (nematistatic effect)
    • Mortality (nematicidal effect)

Table 1. The effect of Cedroz on nematode mobility and mortality

  After 24 hr with Cedroz
[c] Immobile After 24 hr in water
mL/L Staticity Mobility Immobile (mortality)
2.00 66.67 6.67 60.00
2.50 93.33 0.00 93.33
4.00 90.00 0.00 90.00
5.00 100.00 0.00 100.00


  After 48 hr with Cedroz
[c] Immobile After 24 hr in water
mL/L Staticity Mobility Immobile (mortality)
2.00 73.33 3.33 70.00
2.50 100.00 0.00 100.00
4.00 100.00 0.00 100.00
5.00 100.00 0.00 100.00


Cedroz has shown a prevalent nematicidal activity against J2 of the root-knot nematode Meloidogyne incognita in all in vitro bioassays.

The soil cross-section reveals growing plants with deep roots

Pot trials

A. Infectivity of J2s exposed to Cedroz

Step 1: 400 M. incognita juveniles (J2) were treated with the LD50 of Cedroz (1.5 mL/L); after 24 hours, ± 200 living, moving J2s were recovered.

Step 2: Three-week-old tomato plants (cv. Moneymaker) were inoculated with 200 moving J2s recovered from either Cedroz at its LD50 (1.5 mL/L) or a tap water control.

Twenty-four small plants planted in square nursery pots

Figure 1. Cedroz vs. control galls per root

Graph shows that Cedroz-treated tomato plants have less than half the number of galls per root system compared to control

Figure 1 illustrates that tomato plants treated with Cedroz exhibited fewer than half the number of galls per root system compared to the control group.


Nematodes that survived Cedroz exposure were much less infectious, as indicated by the number of galls after three weeks (p < 0.001, N = 6).

B. Activity on mass eggs on RKN (Dr. Curto)

Cedroz in contact with the egg masses for a period of seven days proved capable of inhibiting the hatching of second-age J2 larvae. Therefore, there was a temporary inhibition in the presence of the solution with Cedroz or due to the inability of the product to penetrate the eggs. Further studies are underway to check the activity on the individual egg.

Table 2. Results of the activity of Cedroz on the hatching of eggs contained in the egg sack of Meloidogyne incognita

After 7 days
  Total J2 mobile J2 immobile
  a b c
10 0.00 0.00 0.00
5 4.75 1.25 3.50
2.5 37.75 24.75 13.00
1.25 54.00 47.25 6.75
0.625 110.00 108.75 1.25
0.315 112.75 112.50 0.25
0 104.50 104.25 0.25


After 7 days in water
n° eggs n° J2
    Total L2 mobile L2 immobile
  d e f g
10 84.25 41.40 35.75 5.75
5 43.00 53.00 51.75 1.25
2.5 50.00 87.00 83.50 3.50
1.25 15.25 83.25 79.25 4.00
0.625 58.00 70.25 66.25 4.00
0.315 40.75 51.00 51.00 0.00
0 36.25 12.75 12.75 0.00


n° eggs/egg sack % hatched
  (a+d+e) After 7 days After 14 days
  h A*100/h (a+e)*100/h
10 125.75 0.00 33.00
5 100.75 4.71 57.32
2.5 174.75 21.60 71.39
1.25 152.50 35.41 90.00
0.625 238.25 46.17 75.66
0.315 204.50 55.13 80.07
0 153.50 68.08 76.38
Ripe, red strawberries hanging off the vine

Mode of action

Cedroz inactivates cellular membranes of root-knot nematodes through different mechanisms.

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Uncover nature’s secret

Cedroz is made of two unique active ingredients that can be found in nature. Learn more about these compounds and how they work.

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A farmer picking an orange pepper from the pepper plant
Close-up of root knots on a plant caused by root-knot nematodes

Innovative delivery

The unique formulation technology of Cedroz ensures strategic and effective delivery to combat root-knot nematodes. Discover the mechanism of product release for optimal results.

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